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KMID : 1149220130210010154
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Korean Journal of Oriental Medical Prescription
2013 Volume.21 No. 1 p.154 ~ p.160
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Inhibitory Effect of Rhein on Renal Fibrosis in Diabetic Nephropathy Rats
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Rongjie Zhao
Zhenglin Zhao
Jie Zhang
Hongfeng Liu
Rongjun Cui
Kim Sang-Chan
Kim Sun-Hyung
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Abstract
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Objectives : To investigate the therapeutic effect and underlying mechanisms of rhein on renal fibrosis in diabetic rats.
Methods : Diabetic nephropathy (DN) was induced in adult Wistar rats via introperitoneal injection of streptozotocin (STZ) (20 mg/kg/d) for three consecutive days. Two days after the last dose of STZ, rhein was administered to the diabetic rats at a dose of 25 mg/kg or 50 mg/kg, twice a day by gavage, respectively. Following 28 days treatment with rhein, the plasma glucose and creatinine levels were measured, the renal levels of TGF-β1 protein and mRNA were examined, and the fibronectin mRNA levels were also determined.
Results : Rhein significantly inhibited the increased plasma glucose and creatinine levels of diabetic rats in a dose- and a time-dependent way. Immunohistochemical analysis showed both doses of rhein markedly attenuated elevated induction of renal TGF-β1 protein expressions in diabetic rats. Additionally, the high dose of rhein improved both TGF-β1 and fibronectin mRNA expressions, while the low dose of rhein only alleviated fibronectin mRNA expressions.
Conclusions : Rhein can improve renal fibrosis in diabetic nephropathy rats, and which may be mediated through inhibition of the renal mRNA expressions of TGF-β1and fibronectin.
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KEYWORD
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Rhein, Diabetic Renal Fibrosis, TGF-β1, Fibronectin
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